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El grupo de investigación Lipids & Liver del departamento de Fisiología de la Facultad de Medicina y Enfermería de la UPV/EHU trabaja en la búsqueda de los mecanismos implicados en el desarrollo y la progresión de las enfermedades hepáticas, y principalmente mecanismos relacionados con alteraciones vinculadas al metabolismo del lípido (grasa). En concreto, una de las líneas de investigación del grupo, focalizada en el estudio de la enfermedad del hígado graso no alcohólica, se centra en investigaciones que tienen el fin de “saber por qué evoluciona la enfermedad en unos pacientes y en otros no; encontrar marcadores de evolución, que nos puedan dar un pronóstico de en qué pacientes va a evolucionar la enfermedad; y encontrar marcadores no solo hepáticos (que se pueden obtener mediante biopsia), sino además marcadores séricos, que mediante simples análisis de sangre se pueda decir la fase en la que se encuentra el paciente”, explica la doctora Patricia Aspichueta, responsable de la línea de investigación.
Básicamente, los miembros del grupo de investigación se centran en el estudio del metabolismo de los lípidos en el hígado, es decir, en las reacciones químicas en las que se forman o se consumen. Para ello, trabajan con modelos animales a los que se ha silenciado algún gen y a los que se administran diferentes dietas y tratamientos inductores de la enfermedad, y una vez que validan o identifican alguna diana en el modelo animal, pasan a validarla en muestras humanas. Además, también trabajan con modelos in vitro o celulares, “porque allí es más sencillo saber en qué célula está dañado, modificado o alterado el metabolismo, y podemos jugar con diferentes fármacos, diferentes inductores, y es más fácil silenciar determinadas vías metabólicas, etc.”, explica Aspichueta.
Los miembros del grupo estudian distintas moléculas o dianas implicadas en diferentes procesos de la enfermedad hepática. Aspichueta especifica algunas de las vías que tienen abiertas en el grupo de investigación: “Por una parte, queremos identificar proteínas que modifican las vías metabólicas y que aportan más lípido al hígado. Queremos saber por qué se forma ese almacén de lípido, y por qué ese almacén hace progresar a la enfermedad, incluso a fases como el cáncer. Es importante saber quiénes son los actores de esos procesos, para poder silenciarlos y ver si la enfermedad revierte”. Además, investigan la implicación que tienen los lípidos en la regeneración hepática, “un punto de vista muy importante de cara a los pacientes a los que se extirpa un trozo de hígado, porque el hígado es el único órgano que vuelve a recuperar su tamaño normal”. En otro de los estudios investigan “cómo el hígado controla el tejido adiposo, y cómo modulando el funcionamiento del hígado conseguimos hacer a los animales adelgazar”.

En el contexto de cambio climático actual, es cada vez más importante mantener los programas existentes de monitorización del ócéano a largo plazo, debido a que, entre otros motivos, dichos programas de monitorización suponen el único medio para poder analizar y cuantificar de forma precisa la variabilidad del clima oceánico, para poder comprobar si se producen cambios fuera de lo habitual y para determinar si tales cambios podrían ser de origen natural y/o debidos a la propia acción del ser humano.
Este tipo de observaciones de largo plazo son de gran interés ya que permiten calcular estados promedio a partir de los cuales se pueden hacer distinciones cuantificadas y precisas entre condiciones medias o normales en el clima oceánico y condiciones anómalas o fuera del rango. Además de la temperatura y salinidad, se miden otras variables complementarias como pueden ser la transmisión de luz, el contenido de oxígeno disuelto, o la concentración de clorofila desde superficie a fondo.
En el golfo de Bizkaia no existen muchas localizaciones donde se lleven a cabo mediciones de forma rutinaria y que abarquen la columna de agua desde la superficie hasta el fondo. Entre las series existentes, la más antigua está localizada en la costa de Pasaia, donde AZTI lleva 32 años realizando mediciones que han permitido calcular que la temperatura media desde la superficie del mar hasta el fondo se ha incrementado casi medio grado desde 1986. En la actualidad, las mediciones se realizan sobre fondos de 110 metros de profundidad como máximo, sin embargo, dada la afección que el cambio climático puede producir en nuestra costa, sería necesario realizar mediciones a mayor profundidad, ya que se desconoce lo que está ocurriendo por debajo de las profundidades señaladas.

El Ministerio de Ciencia, Innovación y Universidades ha reconocido a CIC biomaGUNE como Unidad de Excelencia “María de Maeztu”, el mayor reconocimiento de unidades de excelencia en España. Este distintivo tiene como objetivo financiar y acreditar unidades de investigación que demuestran impacto y liderazgo científico a nivel internacional y que colaboran activamente en su entorno social y empresarial.
Las unidades de excelencia María de Maeztu son centros o estructuras organizativas que cuentan con programas de investigación altamente competitivos, situados entre los mejores del mundo en sus respectivas áreas científicas.
El proceso de evaluación y selección se lleva a cabo de forma independiente por un comité científico internacional, integrado por investigadores de reconocido prestigio.
La acreditación tiene una validez de cuatro años, con posibilidad de renovación, e implica la concesión de una ayuda de dos millones de euros para este periodo. CIC biomaGUNE destinará esta inversión al fortalecimiento de la investigación aplicada, a la generación de conocimiento fundamental y orientado en biomateriales, así como a la formación de talento y a la mejora de su visibilidad pública y social.
Para optar a este reconocimiento, CIC biomaGUNE ha demostrado cumplir con requisitos de excelencia, que se caracterizan por un alto impacto y nivel de competitividad en su campo de actividad, en el escenario científico mundial. El centro somete periódicamente sus actividades de investigación a procesos de evaluación científica mediante un comité científico externo e independiente; desarrolla sus actividades de investigación conforme a un programa estratégico que en la frontera del conocimiento; realiza actividades de formación, selección y atracción de recursos humanos a nivel internacional; mantiene acuerdos activos de colaboración e intercambio a nivel institucional con otros centros de investigación de alto nivel; y potencia las actividades de transferencia y divulgación del conocimiento a la sociedad.
Las Unidades de Excelencia “María de Maeztu” se distinguen, asimismo, por contar con un director científico –Luis Liz Marzán, en el caso de CIC biomaGUNE–, reconocido en el mundo de la ciencia según los estándares más exigentes y, al mismo tiempo, con una importante capacidad de liderazgo en la organización de la investigación.

Es habitual añadir al mortero u hormigón diferentes tipos de fibras, para dotarlo de mayor durabilidad, resistencia y firmeza. Por evitar la fabricación de estos aditivos, un investigador del Departamento de Ingeniería Mecánica de la UPV/EHU, junto con las universidades chilenas Bio-Bio y Andres Bello, ha recurrido a los residuos de acero industriales, en forma de fibras y virutas, y determinado cómo cambian las propiedades del compuesto resultante, en función de las cantidades añadidas.
En la actualidad, existe una gran variedad de morteros en el mercado que llevan incorporadas fibras de plástico, carbono y metal, entre otras, como refuerzo. “En nuestra investigación, hemos querido utilizar fibras que no han pasado por un proceso de fabricación, y hemos recurrido a residuos de acero, en forma de fibras virutas, generados en la industria. De esta forma, conseguimos, por un lado, evitar la propia fabricación de las fibras, y, por otro, reutilizar directamente los residuos industriales, sin someterlos a ningún proceso de reciclado”, comenta Roque Borinaga Treviño, investigador del Departamento de Ingeniería Mecánica de la UPV/EHU y uno de los autores de este trabajo.
En la investigación también quisieron verificar si había opción de realizar la monitorización de las estructuras reforzadas con residuos de acero. En anteriores estudios se ha podido demostrar que la monitorización de las propiedades eléctricas y térmicas permite detectar, por ejemplo, grietas en las estructuras. “En el caso de haber alguna grieta, sucederá una desconexión entre las fibras, lo cual modificará las propiedades eléctricas y térmicas del mortero. Por tanto, en las mediciones realizadas mediante distintos dispositivos detectaríamos unas diferencias mayores”, explica el Dr. Borinaga.
El estudio consistió en hacer el seguimiento de la forma en que cambian las propiedades eléctricas y térmicas en función del tipo y la cantidad de residuo utilizadas. Para la parte de las propiedades eléctricas, tomaron como indicador de la monitorización la resistividad eléctrica del mortero. El mortero se caracteriza por tener una resistividad eléctrica grande, es decir, la electricidad tiene una capacidad muy limitada para atravesarlo. Las fibras, sin embargo, hacen que esa resistividad sea menor. En cuanto a las propiedades térmicas, el indicador elegido fue la conductividad térmica. La conductividad térmica del acero es 15-20 veces mayor que la del mortero, por lo que un mortero convencional presentará una resistencia mayor a que el calor pase a través suyo que uno reforzado con fibras de acero.

NEIKER-Tecnalia ha desarrollado un método pionero que permite cuantificar las emisiones del ganado vacuno mediante técnicas no invasivas basadas en la medida de la concentración de metano en el aire exhalado a través de la eructación. Esta solución permite obtener información de utilidad acerca de las emisiones totales diarias de metano de cada vaca en condiciones comerciales.

Este innovador método es clave en el proyecto Metalgen, que pretende mejorar la eficiencia alimentaria y reducir las emisiones de metano en la raza frisona lechera, contribuyendo a mantener sostenible la huella de carbono de la producción láctea.

NEIKER-Tecnalia dispone de dos aparatos medidores de la concentración de metano, que se han colocado en cuatro explotaciones de Euskadi, recogiendo las emisiones individuales de metano de más de 400 vacas. De esta forma, ha conseguido obtener los primeros datos medidos reales a nivel estatal de la cabaña ganadera del vacuno lechero. El proyecto continuará con el muestreo de la cabaña ganadera en Euskadi y en el Estado. Hasta la fecha hay 13 explotaciones ganaderas dispuestas a participar en el desarrollo del proyecto.

La principal ventaja de esta técnica basada en el análisis del aire exhalado es que permite, de forma sencilla y barata, la toma de muchos datos sin interferir en el comportamiento habitual de los animales ni en la producción, estando alineadas con los requerimientos de bienestar de los animales. Hasta ahora, el método de referencia utilizado a nivel experimental es la cámara metabólica, cuyo uso a nivel de explotación comercial es inviable.

El proyecto Metalgen se propone resolver dos problemas relacionados entre sí como son la eficiencia alimentaria y las emisiones de metano debidas a la fermentación entérica de los rumiantes, un proceso digestivo que es exclusivo de este tipo de animales. Se pretende establecer los primeros pasos para utilizar la selección genética con el objetivo de interferir en el microbioma ruminal. La flora microbiana del rumiante tiene una gran influencia sobre la digestión del alimento y sus características están, a su vez, determinadas por la alimentación y el genotipo del animal.

NEIKER-Tecnalia participa en el proyecto junto a la Confederación de Asociaciones de Frisona Española (CONAFE) en este proyecto liderado por el Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA).

La mejora de la eficiencia alimentaria y la mitigación de emisiones de gases con efecto invernadero son unas de las nuevas y principales preocupaciones en el sector ganadero en la Unión Europea por lo que la investigación en genética y nutrición es un factor clave para reducir la emisión de gases de efecto invernadero, principales causantes del cambio climático.